- ￥1560
- Sino Biological
- 北京
- 90311-C08HL
- 2025年08月12日
- WB
- HEK293 Cells
- Cynomolgus
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
A DNA sequence encoding the cynomolgus PDCD1 (NP_001271065.1) (Met1-Gln167) was expressed with a polyhistidine tag at the C-terminus.
- 亚型：
见说明书
- 形态：
液体
- 保存条件：
4℃
- 克隆性：
无
- 标记物：
见说明书
- 适应物种：
Cynomolgus
- 保质期：
12个月
- 抗原来源：
见说明书
- 目录编号：
90311-C08HL
- 级别：
免疫学
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 宿主：
HEK293 Cells
- 应用范围：
WB
- 浓度：
见说明书
- 靶点：
PDCD1
- 抗体英文名：
Cynomolgus PD1 / PDCD1 / CD279 CHO Cell Lysate (WB positive control)
- 抗体名：
Cynomolgus PD1 / PDCD1 / CD279 CHO Cell Lysate (WB positive control)
- 规格：
300 µg
反应种属：Cynomolgus
裂解液靶点：PDCD1
裂解液应用：WB
裂解液保存条件：Store at 4℃. After re-dissolution, aliquot and store at -80℃.
裂解液产品描述：HEK293 Cell Cynomolgus PD1 / PDCD1 / CD279 transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer).
裂解液表达宿主：HEK293 Cells
裂解液制备方法：Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
裂解液Buffer：Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
裂解液质控信息：12.5% SDS-PAGE Stained with Coomassie Blue after protein purification.
裂解液使用建议：1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃.
裂解液稳定性：Samples are stable for up to twelve months from date of receipt.
裂解液使用说明：Western blot (WB): Use at an assay dependent dilution. Other Applications: Not tested.
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文献和实验by volume. Note: The Triton-X-100 will not dissolve immediately.It will go into solution slowly over night. Stir gently over night in the 4℃ walk-in. Transfer the liquid to centrifuge bottles and spin down lysate at 8000g for 1 hour. Decant lysate in a clean
, macrophages, myeloid cells beginning with promyelocytes, NK cells, some B/T cells Positive staining (disease): AML-M1-M3 (35-70%), M4-M5 (80-90%); hairy cell leukemia (virtually all) CD11c Aka CR4, iC
DNA/RNA/Protein Purification from Cultured Cells Using SQ DNA/RNA/Protein Cell Kit (1-2 x 106 cells)
of the reagent per 2 x 106 of cultured cells. 2) Cells Grown in Monolayer Lyse cells directly in a culture dish by adding 300 μL of Cell Lysis Buffer per 2 x 106 of cultured cells directly into each well of multiwell cell culture plate or flask
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