- ￥1560
- Sino Biological
- 北京
- 90299-C08HL
- 2025年08月12日
- WB
- HEK293 Cells
- Cynomolgus
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
A DNA sequence encoding the cynomolgus EPCAM (XP_005576740.1) (Met1-Lys265) was expressed with a polyhistidine tag at the C-terminus.
- 亚型：
见说明书
- 形态：
液体
- 保存条件：
4℃
- 克隆性：
无
- 标记物：
见说明书
- 适应物种：
Cynomolgus
- 保质期：
12个月
- 抗原来源：
见说明书
- 目录编号：
90299-C08HL
- 级别：
免疫学
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 宿主：
HEK293 Cells
- 应用范围：
WB
- 浓度：
见说明书
- 靶点：
EpCAM
- 抗体英文名：
Cynomolgus EpCAM / TROP-1 / TACSTD1 HEK293 Cell Lysate (WB positive control)
- 抗体名：
Cynomolgus EpCAM / TROP-1 / TACSTD1 HEK293 Cell Lysate (WB positive control)
- 规格：
300 µg
反应种属：Cynomolgus
裂解液靶点：EpCAM
裂解液应用：WB
裂解液保存条件：Store at 4℃. After re-dissolution, aliquot and store at -80℃.
裂解液产品描述：Human Cell lysate that Cynomolgus EPCAM / TROP1 transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer).
裂解液表达宿主：HEK293 Cells
裂解液制备方法：Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
裂解液Buffer：Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
裂解液质控信息：12.5% SDS-PAGE Stained with Coomassie Blue after protein purification.
裂解液使用建议：1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
裂解液稳定性：Samples are stable for up to twelve months from date of receipt.
裂解液使用说明：Western blot (WB): Use at an assay dependent dilution. Other Applications: Not tested. Optimal dilutions/concentrations should be determined by the end user.
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文献和实验Cell death detection in Xenopus embryos by ELISA
a negative control, as shearing of genomic DNA may give false positive signals Dilute 10 µl lysate with 190 µl "incubation buffer" Consider testing other dilutions Incubate this sample for 30 min. at 4°C Centrifuge at 13,000 rpm
Competent cells, calcium chloride method, E. coli, description
(4000-5000 rpm for 5 min or 5000 x g for 5 min) at 4 C Keep the cells ice cold in all further steps. Resuspend the cells in 1/2 culture volume of 0.1 M ice-cold CaCl2. Hold on ice for minimum of 30 min, prefably 1 - 2 h
quadrants, remove the plate and count the plaques. Calculate the titer of the phage lysate by the following formula (dilution in this case is expressed with a positive exponent): no. of plaques/vol. plated (ml)] x dilution = pfu/mL lysate
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