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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
A DNA sequence encoding the human CROT (Q9UKG9) (Met 1-Leu 612, 474 Leu/Val) was fused with a polyhistidine tag at the C-terminus.
- 亚型:
见说明书
- 形态:
液体
- 保存条件:
4℃
- 克隆性:
无
- 标记物:
见说明书
- 适应物种:
Human
- 保质期:
12个月
- 抗原来源:
见说明书
- 目录编号:
11015-H08BL
- 级别:
免疫学
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 宿主:
Baculovirus-Insect cells
- 应用范围:
WB
- 浓度:
见说明书
- 靶点:
CROT
- 抗体英文名:
Human CROT (474 Leu/Val) Insect Cell Lysate (WB positive control)
- 抗体名:
Human CROT (474 Leu/Val) Insect Cell Lysate (WB positive control)
- 规格:
300 µg
反应种属:Human
裂解液靶点:CROT
裂解液应用:WB
裂解液保存条件:Store at 4℃. After re-dissolution, aliquot and store at -80℃.
裂解液产品描述:Baculovirus-Insect Cell lysate that Human CROT transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer).
裂解液表达宿主:Baculovirus-Insect cells
裂解液制备方法:Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
裂解液Buffer:Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
裂解液质控信息:12.5% SDS-PAGE Stained with Coomassie Blue after protein purification.
裂解液使用建议:1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
裂解液稳定性:Samples are stable for up to twelve months from date of receipt.
裂解液使用说明:Western blot (WB): Use at an assay dependent dilution. Other Applications: Not tested. Optimal dilutions/concentrations should be determined by the end user.
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文献和实验北京义翘神州生物技术有限公司(Sino Biological Inc.)--致力于为全球生命科学研究人员提供高质量的重组蛋白、抗体等科研工具试剂。 • 国际一流的团队:4名中央"千人计划",300人科研团队 • 一流的技术平台:蛋白表达大规模生产,鼠单抗与兔单抗技术 • 国内最大蛋白、抗体库:2000多种蛋白,1500多种抗体 • 抗体检测限比国际知名品牌抗体低10~160倍 • 3000多家全球客户:哈佛医学院、斯坦
8 阳性对照 0.5ml × 1 瓶 3 酶标包被板 12 孔× 8 条 9 阴性对照 0.5ml ×
Function),生物过程(biological process)和细胞组成(cellular component)三个部分。蛋白质或者基因可以通过ID对应或者序列注释的方法找到与之对应的GO号,而GO号可对于到Term,即功能类别或者细胞定位。 功能富集分析: 功能富集需要有一个参考数据集,通过该项分析可以找出在统计上显著富集的GO Term。该功能或者定位有可能与研究的目前有关。 GO功能分类是在某一功能层次上统计蛋白或者基因的数目或组成,往往是在GO的第二层次。此外也有研究都挑选一些Term
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