- ￥1560
- Sino Biological
- 北京
- 10156-H02HL
- 2025年08月12日
- WB
- HEK293 Cells
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
A DNA sequence encoding the extracellular domain (Met 1-Glu 356) of human IL1R9 (NP_059112.1) precursor was expressed with the fused Fc region of human IgG1 at the C-terminus.
- 亚型：
见说明书
- 形态：
液体
- 保存条件：
4℃
- 克隆性：
无
- 标记物：
见说明书
- 适应物种：
Human
- 保质期：
12个月
- 抗原来源：
见说明书
- 目录编号：
10156-H02HL
- 级别：
免疫学
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 宿主：
HEK293 Cells
- 应用范围：
WB
- 浓度：
见说明书
- 靶点：
IL1RAPL2
- 抗体英文名：
Human IL-1R9 / IL1RAPL2 HEK293 Cell Lysate (WB positive control)
- 抗体名：
Human IL-1R9 / IL1RAPL2 HEK293 Cell Lysate (WB positive control)
- 规格：
300 µg
反应种属：Human
裂解液靶点：IL1RAPL2
裂解液应用：WB
裂解液保存条件：Store at 4℃. After re-dissolution, aliquot and store at -80℃.
裂解液产品描述：Human Cell lysate that Human IL1R9 transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer).
裂解液表达宿主：HEK293 Cells
裂解液制备方法：Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
裂解液Buffer：Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
裂解液质控信息：12.5% SDS-PAGE Stained with Coomassie Blue after protein purification.
裂解液使用建议：1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
裂解液稳定性：Samples are stable for up to twelve months from date of receipt.
裂解液使用说明：Western blot (WB): Use at an assay dependent dilution. Other Applications: Not tested. Optimal dilutions/concentrations should be determined by the end user.
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文献和实验整个抗体市场的信任危机，早在多年前就未雨绸缪，展开了一些列对于抗体特异性的探索和研发- UltraMAB® 超高特异性抗体就应运而生。OriGene UltraMAB® 超高特异性抗体采用独特的 17k 蛋白芯片验证，OriGene 高密度蛋白芯片上包含 17,000 个 HEK293T 细胞蛋白过表达裂解物，每种蛋白裂解液在芯片上具有两个拷贝的重复。蛋白裂解液被印迹在硝酸纤维素膜上。每一钟蛋白裂解液的定位可以通过 Excel 文件进行准确定位。蛋白芯片上蛋白分为 40 个亚矩阵，每个亚矩阵上有
Background Information of RNAi
that chemically synthesized 21 nt siRNA duplexes specifically suppress the expression of endogenous and heterologeous genes in different mammalian cell lines, including human 293 and HeLa cells (8). A key discovery from these studies was that no non-specific gene
that chemically synthesized 21 nt siRNA duplexes specifically suppress the expression of endogenous and heterologeous genes in different mammalian cell lines, including human 293 and HeLa cells (8). A key discovery from these studies was that no non-specific gene
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