Mouse GM-CSF/CSF2 ELISA Kit(产品说明)
反应种属:小鼠
试剂盒靶点:CSF2
试剂盒应用:ELISA
试剂盒保存:Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA
试剂盒介绍:For the quantitative determination of Mouse GM-CSF/CSF2 concentration in serum and cell culture supernates.
试剂盒线性范围:1.88-120
试剂盒包被抗体类型:rabbit mAb
试剂盒检测抗体类型:rabbit pAbKIT51048
试剂盒检测灵敏度:0.83
试剂盒天然样本检测量:The average concentration of Mouse GM-CSF in 10 normal mouse serum is 22.85 +/- 23.50 pg/mL ranging from ND to 78.39 pg/mL.EL-4 mouse lymphoblast cells (1 x 10E6 cells/mL) were cultured in DMEM supplemented with 10% fetal calf serum,stimulated with 10 μg/mL PHA and 10 ng/mL PMA for 2-3 days.Aliquots of the cell culture supernates were removed and assayed for levels of natural GM-CSF, and measured 1.64 ng/mL.
试剂盒特异性:This assay recognizes both recombinant and natural mouse GM-CSF. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
试剂盒检测原理:Solid Phase Sandwich ELISA
试剂盒组成:1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
试剂盒检测样品:血清,细胞上清液