- ￥2800 - 6800
- Sino Biological
- 46.9 pg/ml
- 北京
- SEK50389
- 2025年08月12日
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- 详细信息
- 技术资料
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 检测范围：
46.9 pg/ml
- 检测方法：
ELISA
- 应用：
ELISA
- 适应物种：
Mouse
- 标记物：
HRP
- 样本：
见说明书
- 规格：
5 Plates/15 Plates
| 规格： | 5 Plates | 产品价格： | ￥2800.0 |
|---|---|---|---|
| 规格： | 15 Plates | 产品价格： | ￥6800.0 |
Mouse CD8a ELISA Pair Set（产品说明）
反应种属：Mouse
试剂对靶点：CD8A
试剂对应用：ELISA
试剂对保存条件：Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -80℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
试剂对线性范围：46.88-3000
试剂对标记类型：HRP
包被抗体：0.5 mg/mL of rabbit anti-mouse CD8a monoclonal antibody. Dilute to a working concentration of 2 μg/mL in CBS before coating. (Catalog: # 50389-R016)
检测抗体：0.4 mg/mL rabbit anti-mouse CD8a polyclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use.
试剂灵敏度：The minimum detectable dose of Mouse CD8a was determined to be approximately 46.9 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
试剂对检测原理： The Mouse CD8a ELISA Pair Set is for the quantitative determinationof Mouse CD8a. This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for CD8a coated on a 96-well plate. Standards and samples are added to the wells, and any CD8a present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-CD8a polyclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of CD8a present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
试剂对检测限：46.9 pg/ml
反应种属：Mouse
试剂对靶点：CD8A
试剂对应用：ELISA
试剂对保存条件：Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -80℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
试剂对线性范围：46.88-3000
试剂对标记类型：HRP
包被抗体：0.5 mg/mL of rabbit anti-mouse CD8a monoclonal antibody. Dilute to a working concentration of 2 μg/mL in CBS before coating. (Catalog: # 50389-R016)
检测抗体：0.4 mg/mL rabbit anti-mouse CD8a polyclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use.
试剂灵敏度：The minimum detectable dose of Mouse CD8a was determined to be approximately 46.9 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
试剂对检测原理： The Mouse CD8a ELISA Pair Set is for the quantitative determinationof Mouse CD8a. This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for CD8a coated on a 96-well plate. Standards and samples are added to the wells, and any CD8a present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-CD8a polyclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of CD8a present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
试剂对检测限：46.9 pg/ml
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Mouse CD8a ELISA Pair Set
￥2800 - 6800
