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- 详细信息
- 文献和实验
- 技术资料
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 检测范围:
11.72
- 检测方法:
Solid Phase Sandwich ELISA
- 应用:
ELISA
- 适应物种:
Human
- 标记物:
见说明书
- 样本:
血清,细胞上清液,尿样
- 规格:
1 Kit (96 Tests)
反应种属:人
试剂盒靶点:CSF1
试剂盒应用:ELISA
试剂盒保存:Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA
试剂盒介绍:For the quantitative determination of Human M-CSF/CSF1 concentration in serum, cell culture supernates and urine.
试剂盒线性范围:11.72-750
试剂盒包被抗体类型:rabbit mAb
试剂盒检测抗体类型:rabbit mAbKIT11792
试剂盒检测灵敏度:11.72
试剂盒天然样本检测量:The average concentration of Human MCSF in 10 normal human serum is 1.47 +/- 0.50 ng/mL ranging from 0.94 to 2.54 ng/mL.The average concentration of MCSF in 4 normal human urine is 0.30 +/- 0.48 ng/mL ranging from 0.016 to 1.01 ng/mL. Human peripheral blood mononuclear cells (1 x 10E6 cells/mL) were cultured in RPMI supplemented with 10% fetal calf serum, 50 μM β-mercaptoethanol,2 mM L-glutamine, 100 units/mL penicillin, 100 μg/mL streptomycin sulfate, stimulated with 10 μg/mL PHA for 5 days. A
试剂盒特异性:This assay recognizes both recombinant and natural Human MCSF. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
试剂盒检测原理:Solid Phase Sandwich ELISA
试剂盒组成:1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
试剂盒检测样品:血清,细胞上清液,尿样
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文献和实验34+、CD45RA low、CD77low的CFU-GM(70倍)和BFU-E(55倍)的最好因子组合分别为SCF/IL-6/PIXY/M-CSF和SCF/IL-6/IL-3/EPO,扩增两者的最佳组合是SCF/IL-6/PIXY/M-CSF/G-CSF/EPO[14]。NakahataT[15]指出,早期HSC大部分无IL-6R表达,IL-6/Sil-6R复合体激发HSC表达gp130,启动HSC自我复制的信号传递,而c-Kit/SCF是HSC自我自制扩增的另一信号传递途径。无血清条件下,IL
【申请上传】Current Protocols in Immunology
Measurement of Soluble and Membrane-Bound Interleukin 1 Using a Fibroblast Bioassay Unit 6.3 Measurement of Human and Murine Interleukin 2 and Interleukin 4 Unit 6.4 Measurement of Interleukin 3 and Other Hematopoietic Cytokines, such as GM-CSF, G-CSF, M-CSF
人细胞因子 >>> 分装ELISA 试剂盒 货号 品名 方法 规格 价格 备注 F0001 人血管紧张素转化酶(Human ACE) ELISA 48T/96T 2000/3000 进口分装 F0002 激活素A(Activin A) ELISA 48T/96T 2000/3000 进口分装 F0003 ★ 人脂联素(human Adiponectin) ELISA 48T/96
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