Human NGF/NGFB/beta-NGF ELISA Kit | 人 NGF/NGFB/beta-NGF 酶联免疫试剂盒

Human NGF/NGFB/beta-NGF ELISA Kit（产品说明）
反应种属：人
试剂盒靶点：NGF
试剂盒应用：ELISA
试剂盒保存：Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA
试剂盒介绍：For the quantitative determination of Human NGF/NGFB/beta-NGF concentration in cell culture supernates.
试剂盒线性范围：3.13-200
试剂盒包被抗体类型：rabbit mAb
试剂盒检测抗体类型：rabbit mAbKIT11050
试剂盒检测灵敏度：1.81
试剂盒天然样本检测量：Human peripheral blood mononuclear cells （1 x 10E6 cells/mL） were cultured in RPMI supplemented with 10% fetal calf serum, 50 μM β-mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate, stimulated with 10 μg/mL PHA for 5 days. Aliquots of the cell culture supernates were removed and assayed for levels of natural β-NGF,The sample measured less than the lowest β-NGF standard,3.13 pg/mL..
试剂盒特异性：This assay recognizes both recombinant and natural Human β-NGF. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed. High cross-reactivity were observed within standard curve of recombinant mouse β-NGF.
试剂盒检测原理：Solid Phase Sandwich ELISA
试剂盒组成：1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
试剂盒检测样品：细胞上清液