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- 详细信息
- 文献和实验
- 技术资料
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 检测范围:
4.69-300
- 应用:
ELISA
- 适应物种:
人
- 样本:
细胞上清液
- 规格:
1 Kit (96 Tests)
反应种属:人
试剂盒靶点:NGF
试剂盒应用:ELISA
试剂盒保存:Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA
试剂盒介绍:For the quantitative determination of Human NGF/NGFB/beta-NGF concentration in cell culture supernates.
试剂盒线性范围:3.13-200
试剂盒包被抗体类型:rabbit mAb
试剂盒检测抗体类型:rabbit mAbKIT11050
试剂盒检测灵敏度:1.81
试剂盒天然样本检测量:Human peripheral blood mononuclear cells (1 x 10E6 cells/mL) were cultured in RPMI supplemented with 10% fetal calf serum, 50 μM β-mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate, stimulated with 10 μg/mL PHA for 5 days. Aliquots of the cell culture supernates were removed and assayed for levels of natural β-NGF,The sample measured less than the lowest β-NGF standard,3.13 pg/mL..
试剂盒特异性:This assay recognizes both recombinant and natural Human β-NGF. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed. High cross-reactivity were observed within standard curve of recombinant mouse β-NGF.
试剂盒检测原理:Solid Phase Sandwich ELISA
试剂盒组成:1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
试剂盒检测样品:细胞上清液
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文献和实验目前自动洗板机应用比较广泛,凡是具有酶联免疫吸附技术(ELISA)的酶反应板均可使用。应用范围有:肿瘤标记物的检测、传染性疾病的检查、人类免疫缺陷病毒(HIV)抗体的检测、沙眼衣原体感染的血清学检查和TORCH感染的血清学检查等。应用ELISA完成这些方面的检测后,使用先进的全自动洗板机进行洗板,以保证测试的准确性和提高实验的可靠性。 自动洗板机是进行酶联免疫实验时,检测各种感染性病毒所必备的设备之一,其作用是通过清洗,实现酶联免疫实验过程中结合相与游离相的分离。当前,酶标洗板
Scanning the human genome with combinatorial transcription factor libraries Published online: 18 February 2003, doi:10.1038/nbt794 March 2003 Volume 21 Number 3 pp 269 - 274 Pilar Blancafort, Laurent Magnenat & Carlos F
的酶活力直接反映游离的酶标记物。均相EIA在临床检验中较少应用。非均相EIA需先进行游离的和结合的标记物的分离。如前所述,固相载体可用作一种分离手段。这种固相酶免疫测定方法在1971年最初建立时称为酶联免疫吸附剂测定(enzyme linked immunosorbent assay),简称ELISA,在国内有译作酶联免疫吸附试验或酶标,已习用。2、ELISA的原理和类型2.1. ELISA的原理ELISA的基础是抗原或抗体的固相化及抗原或抗体的酶标记。结合在固相载体表面的抗原或抗体仍保持其免疫
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