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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Human respiratory syncytial virus (RSV) Fusion glycoprotein / RSV-F Gene ORF cDNA clone expression plasmid, C-Flag tag
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
1 Unit
基因靶点:RSV-F
反应种属:RSV
应用说明:Stable or Transient mammalian expression
cDNA描述:Full length Clone DNA of Human RSV (subtype A, strain A2) Fusion glycoprotein / RSV-F with C terminal Flag tag.
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文献和实验Two-hybrid analysis of genetic
CYH2.Yeast isolated in an interactor hunt were plated on cycloheximide plates to select those that had lost the original bait plasmid but retained the library plasmid.The resulting strain was then mated with a collection of bait strains,including ones
were then cloned into a yeast expression plasmid that contained the Gal4-Zif268 hybrid transcription factor. In this library, zinc finger 3 (the C-terminal finger) of Zif268 was replaced with zinc-finger sequences amplified from the human genome (Fig. 1A).
Strategies to Optimize Protein Expression in E. coli
Burgess‐Brown, N.A., Sharma, S., Sobott, F., Loenarz, C., Oppermann, U., and Gileadi, O. 2008. Codon optimization can improve expression of human genes in Escherichia coli: A multi‐gene study. Protein Expr. Purif. 59:94‐102
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