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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Purified rat IgG
- 亚型:
mouse IgG2b
- 形态:
Each vial contains 0.1 mg IgG in 0.1 ml (1 mg/ml) of PBS pH7.4 with 0.01% thimerosal. Antibody was purified by Protein-A affinity chromatography.
- 保存条件:
Store at -20°C.
- 克隆性:
单克隆
- 标记物:
见下方详细说明
- 适应物种:
R
- 保质期:
1-2年
- 抗原来源:
Purified rat IgG
- 目录编号:
253242
- 级别:
超纯
- 库存:
大量
- 供应商:
Abbiotec
- 宿主:
Mouse
- 应用范围:
E, WB
- 浓度:
见详细说明信息
- 靶点:
Anti-rat light chain Ig
- 抗体英文名:
Anti-rat light chain Ig Antibody
- 抗体名:
Anti-rat light chain Ig Antibody
- 规格:
0.1 mg
Anti-rat light chain Ig Antibody产品详细介绍:
| 产品名称: | Anti-rat light chain Ig Antibody |
| 说明书: | 【点击查看详细说明及参考图片】 |
| 货号: | 253242 |
| 规格: | 0.1 mg |
| 产品描述: | This monoclonal antibody recognizes rat immunoglobulin light chain. It does not react with mouse IgG. The antibody has been tested with rat IgM, IgG1, IgG2a, IgG2b and IgG2c with kappa chains (not tested with lambda chains). The antibody is conjugated to horseradish peroxydase (HRP) to be used as secondary antibody in ELISA and western blot applications. |
| 克隆类型: | Mouse Monoclonal Antibody, HRP-conjugated Antibody, Secondary Antibody |
| 亚型: | mouse IgG2b |
| 免疫原: | Purified rat IgG |
| 适用物种: | R |
| 应用范围: | E, WB |
| 应用说明: | E: 1:5,000; WB: 1:10,000 |
| 别名: | Immunoglobulin G; kappa light chain |
| 形态: | Each vial contains 0.1 mg IgG in 0.1 ml (1 mg/ml) of PBS pH7.4 with 0.01% thimerosal. Antibody was purified by Protein-A affinity chromatography. |
| 保存条件: | Store at -20°C. Minimize freeze-thaw cycles. Product is guaranteed 6 months from the date of shipment. |
| 参考文献: |
ABBIOTEC产品提供质量保证,提供完善的售后和技术支持,ABBIOTEC产品正品包装:
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文献和实验Myosin Light Chain Preparation
in lyophilizer bottles and freeze in liquid nitrogen.Put on lyophilizer and leave until dry (overnight or over weekend). Day 5 22.Resuspend separate light chain pools 1 and 2 in a small volume of Dialyzing Solution G.Pipette into dialyzing tubing (size #20
for at least 1 year. Number of assays: Sufficient material is supplied for 100 assays based on the protocol below. Materials Required but Not Provided Rat and/or hamster antibody conjugates Phosphate buffered
Purification of Myosin Light Chain Kinase
RPM (4℃).Filter the supernatant through glass wool and discard the pellet (or save for light chain prep).Save 1 ml of supernatant (4℃)for assay.Add pepstatin and TLCK to final concentrations of 1 μg/ml and 40 Og/ml,respectively. Volume of supernatant
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