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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 免疫原:
Purified recombinant fragment of human FOXD3 expressed in E. coli.
- 亚型:
Mouse IgG1
- 形态:
Each vial contains 0.1 ml ascitic fluid with 0.03% sodium azide.
- 保存条件:
Store at -20°C
- 克隆性:
单克隆
- 标记物:
见下方详细说明
- 适应物种:
H
- 保质期:
1-2年
- 抗原来源:
Purified recombinant fragment of human FOXD3 expressed in E. coli.
- 目录编号:
253100
- 级别:
超纯
- 库存:
大量
- 供应商:
Abbiotec
- 宿主:
Mouse
- 应用范围:
WB, IHC
- 浓度:
见详细说明信息
- 靶点:
FOXD3 (5G9)
- 抗体英文名:
FOXD3 (5G9) Antibody
- 抗体名:
FOXD3 (5G9) Antibody
- 规格:
0.1 ml
FOXD3 (5G9) Antibody产品详细介绍:
| 产品名称: | FOXD3 (5G9) Antibody |
| 说明书: | 【点击查看详细说明及参考图片】 |
| 货号: | 253100 |
| 规格: | 0.1 ml |
| 产品描述: | FoxD3 (Forkhead box protein D3) is a transcriptional regulator, a member of the Forkhead Box family. It is characterized by a winged-helix DNA-binding structure and the important role it plays in embryonic development. FoxD3 is required for the maintenance of pluripotency in the pre-implantation and peri-implantation stages of mouse embryonic development and is also required for trophoblast formation. FoxD3 is required for the maintenance of the mammalian neural crest; FoxD3(-/-) mouse embryos fail around the time of implantation with loss of neural crest-derived structures. FoxD3 also forms a regulatory network with Oct-4 and NANOG to maintain the pluripotency of ES cells. Promotes development of neural crest cells from neural tube progenitors. Restricts neural progenitor cells to the neural crest lineage while suppressing interneuron differentiation. Required for maintenance of pluripotent cells in the pre-implantation and peri-implantation stages of embryogenesis. FoxD3 is expressed in chronic myeloid leukemia, Jurkat T-cell leukemia and teratocarcinoma cell lines, but not in any other cell lines or normal tissues examined. |
| 克隆类型: | Mouse Monoclonal Antibody |
| 亚型: | Mouse IgG1 |
| 免疫原: | Purified recombinant fragment of human FOXD3 expressed in E. coli. |
| 适用物种: | H |
| 应用范围: | WB, IHC |
| 应用说明: | WB: 1:500-1:2000; IHC: 1:200-1:1000; |
| 别名: | Forkhead box protein D3; HNF3/FH transcription factor genesis; HFH2; FOXD3 |
| 形态: | Each vial contains 0.1 ml ascitic fluid with 0.03% sodium azide. |
| 保存条件: | Store at 4°C for short term use only. Store at -20°C for storage over 1 month. Product is guaranteed 6 months from the date of shipment. |
| 参考文献: |
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文献和实验Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
are very stable - even in long term large scale cultures (bioreactors). b) High levels of monoclonal antibody secretion in culture - often 100 micrograms per ml of ordinary tissue culture, without recloning, and up to 5 milligrams per ml in hollow fibre
挑选14个克隆进行筛选,结果发现14个克隆均能与靶抗原有反应,其中以4(E4F4)、5(E5F5)、6(G4)、7(G5)、11(G9)克隆颜色较深,挑取6号克隆,利用APAAP法进一步检测其结合活性。结果显示,克隆6的表达产物能和Daudi细胞特异结合。克隆6的测序结果和氨基酸序列分析表明该基因序列完全符合PDB库中抗体所具有的若干保守的框架氨基酸的特点,该序列是一抗体基因序列。2、抗CD20嵌合抗体Fab和F(ab)2的表达与纯化SDS-PAGE电泳结果表明Protein G可有效纯化Fab
【共享】Methods in molecular biology 下载
://mihd.net/9sd6uy Vol 45 Monoclonal Antibody Protocols http://rapidshare.com/files/10764903/045-monoclonal_antibody_protocols.pdf Vol 46 Diagnostic Bacteriology Protocols http://rapidshare.com/files/10765237/046-Diagnostic_Bacteriology_Protocols
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