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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Purified recombinant fragment of human VCAM1 expressed in E. coli.
- 亚型:
Mouse IgG1
- 形态:
Each vial contains 0.1 ml ascitic fluid with 0.03% sodium azide.
- 保存条件:
Store at -20°C
- 克隆性:
单克隆
- 标记物:
见下方详细说明
- 适应物种:
H
- 保质期:
1-2年
- 抗原来源:
Purified recombinant fragment of human VCAM1 expressed in E. coli.
- 目录编号:
253005
- 级别:
超纯
- 库存:
大量
- 供应商:
Abbiotec
- 宿主:
Mouse
- 应用范围:
WB, IHC
- 浓度:
见详细说明信息
- 靶点:
VCAM1 (6G9)
- 抗体英文名:
VCAM1 (6G9) Antibody
- 抗体名:
VCAM1 (6G9) Antibody
- 规格:
0.1 ml
VCAM1 (6G9) Antibody产品详细介绍:
| 产品名称: | VCAM1 (6G9) Antibody |
| 说明书: | 【点击查看详细说明及参考图片】 |
| 货号: | 253005 |
| 规格: | 0.1 ml |
| 产品描述: | VCAM-1 (Vascular cell adhesion molecule 1) is a member of the Ig superfamily that plays an important role in cell-cell recognition. VCAM-1 interacts with beta-1 integrin (VLA4) on leukocytes to mediate adhesion and signal transduction during leukocyte-endothelial cell adhesion. VCAM-1 is likely involved in atherosclerosis and rheumatoid arthritis. |
| 克隆类型: | Mouse Monoclonal Antibody |
| 亚型: | Mouse IgG1 |
| 免疫原: | Purified recombinant fragment of human VCAM1 expressed in E. coli. |
| 适用物种: | H |
| 应用范围: | WB, IHC |
| 应用说明: | WB: 1:500-1:2000; IHC: 1:200-1:1000; |
| 别名: | Vascular cell adhesion protein 1; V-CAM1; INCAM-100; CD106 antigen; VCAM1; L1CAM |
| 形态: | Each vial contains 0.1 ml ascitic fluid with 0.03% sodium azide. |
| 保存条件: | Store at 4°C for short term use only. Store at -20°C for storage over 1 month. Product is guaranteed 6 months from the date of shipment. |
| 参考文献: |
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文献和实验Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
are very stable - even in long term large scale cultures (bioreactors). b) High levels of monoclonal antibody secretion in culture - often 100 micrograms per ml of ordinary tissue culture, without recloning, and up to 5 milligrams per ml in hollow fibre
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论文 Mendelian randomization of genetically independent aging phenotypes identifies LPA and VCAM1 as biological targets for human aging。 该研究将六项大型基因研究结果和人类衰老现象相结合,发现了在衰老中具有负面影响的两种蛋白,即载脂蛋白 a 和血管细胞粘附因子 1。研究结果表明低水平表达这两种蛋白的人更健康,寿命更长! 图 3:来源 Nature Aging 4. Nature
挑选14个克隆进行筛选,结果发现14个克隆均能与靶抗原有反应,其中以4(E4F4)、5(E5F5)、6(G4)、7(G5)、11(G9)克隆颜色较深,挑取6号克隆,利用APAAP法进一步检测其结合活性。结果显示,克隆6的表达产物能和Daudi细胞特异结合。克隆6的测序结果和氨基酸序列分析表明该基因序列完全符合PDB库中抗体所具有的若干保守的框架氨基酸的特点,该序列是一抗体基因序列。2、抗CD20嵌合抗体Fab和F(ab)2的表达与纯化SDS-PAGE电泳结果表明Protein G可有效纯化Fab
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