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- 英文名：
江苏省授权一级代理商
- 库存：
大量
- 供应商：
无锡莱弗思生物实验器材有限公司
- 规格：
25T
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文献和实验E.Z.N.A.® Endo-free Plasmid Mini Kit I Spin Protocol
实验步骤 1. Inoculate 5ml LB/ampicillin (50 ug/ml) medium placed in a 10-20 ml culture tube with E.coli carrying desired plasmid and grow at 37°C with agitation for 12-16 h. It is strongly recommended that an endA negative
E.Z.N.A. Endo-Free Plasmid Mini Kit II Spin Protocol
with occasional mixing. Avoid vigorous mixing as this will shear chromosomal DNA and lower plasmid purity. (Store Solution II tightly capped when not in use.) 4. Add 250 ul ice-cold Buffer N3 and mix gently but throughly by inverting tube several times
Fastfilter Plasmid Midi Kit Spin Protocol
plasmid purity. Prolonged incubation may lead to nicking of plasmid DNA. (Store Solution II tightly capped when not in use.) 5. Add 1.25 ml ice-cold Buffer N3, cover, and gently mix by inverting tube several times until a flocculent white precipitate
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