Toxoplasma IgM ELISA Kit is a sandwich enzyme immunoassay for the detection of Toxoplasma IgM.
Suitable Sample:
Serum
Sample Volume:
100 uL
Label:
HRP-conjugate
Detection Method:
Colorimetric
Intra-Assay:
5.33%
Inter-Assay:
7.60%
Limit of Detection:
95.00%
Reactivity:
Viruses
Storage Instruction:
Store the kit at 4°C.
• A total of 120 patient samples were used to evaluate specificity and sensitivity of the test. The Abnova’s Toxo IgM ELISA test results were compared to a commercial ELISA kit results: Reference Toxo IgM ELISA N E P Total Abnova Toxo IgM ELISA N 71(D) 0 3(B) 74 E 0 0 0 0 P 2(C) 0 44(A) 46 Total 73 0 47 120 Sensitivity = A / (A+B) = 44 / 46 = 95.7% Specificity = D / (C+D) = 71 / 73 = 97.3% Accuracy = (A+D) / (A+B+C+D) = 115 / 120 = 95.8% • Precision: The Precision of the assay was evaluated by testing three different sera of 20 replicates over 4 days. The intra-assay and inter-assay C.V. are summarized below: Negative Low Positive Positive Intra-assay 5.2% 5.0% 5.8% Inter-assay 8.7% 7.2% 6.9%