- ￥4800
- IT-E3Ab
- 2025年08月16日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
研卉生物
- 检测方法：
elisa
- 应用：
elisa检测，体外研究
- 样本：
血浆，血清
- 适应物种：
人
- 规格：
96T
| at No.: | IT-E3Ab-IgG/IgM(H7N9) |
| Source: | |
| Formulation: | |
| Product Type: | Influenza A |
| Protein: | |
| Immunogen: | |
| Applications: | Antibody Detection Kit for Influenza virus H7N9 Hemagglutinin (HA) specific human IgG/IgM is developed for qualitative analysis of anti-H7N9 IgG/IgM concentrations in human serum. |
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文献和实验ELISA Procedure for Measuring Serum Antibody Titer
to the polystyrene microtiter plate first. The antiserum containing the anti-peptide antibody is then added to the well and allowed to bind. Finally, a second antibody, specific for the first antibody and labeled for detection, is added to the well and allowed
, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
. We have developed and optimized laboratory diagnostic techniques, including virus isolation in HRT-18 cell cultures, antibody and antigen ELISA, and RT-PCR, for rapid, sensitive, and reliable diagnosis of BCoV and related wild ruminant coronaviruses.
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