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文献和实验Hybridization of High Density Arrayed BAC Nylon Filter Blots
volume of 30µl, 3µl from each of a group of 10 resuspended insert purifications from above into 1.8ml microfuge tube. Boiling dH2 O bath, 5min. Immediately place on ice. Add 3µl Hexanucleotide Mix (Boehringer Mannheim, 1277081). Add
Kingfisher Flex 96 Plant DNA High Pure Protocol
sample twice during incubation by inverting tube or vortexing plate very briefly. 4. Centrifuge at 3,000-12,000 x g (5,000 x g is better, if available) for 10 min. Compact pellets will form at bottom of tubes but some particles may float. Be careful
for 4 minutes to determine if the DNA is completely in solution. The DNA is not in solution if a clear pellet can be observed. Transfer the top 190µl to a clean 1.5ml microfuge tube, and proceed with shearing. *Note: We have found that the following works
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