- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-52833c
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测鸡血清，血浆等样本中目标蛋白的浓度
- 适应物种：
鸡
- 标记物：
Chicken Rap1 GTPase-activating protein 2,RAP1GAP2
- 样本：
鸡的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Chicken Rap1 GTPase- activating protein 2, RAP1GAP2 ELISA KIT
ELI-52833c
FOR LABORATORY RESEARCH USE ONLY. NOT FOR
USE IN DIAGNOSTIC PROCEDURES.
Chicken Rap1 GTPase- activating protein 2, RAP1GAP2 ELISA KIT is for the quantitative in vitro determination of Chicken RAP1GAP2 concentrations in serum - plasma - tissue homogenates - other biological fluids.
INTENDED USE AND TEST PRINCIPLE
This Chicken Rap1 GTPase- activating protein 2, RAP1GAP2 ELISA KIT is intended Laboratory for Research use only and it is not for use in diagnostic or therapeutic procedures. The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Chicken RAP1GAP2 in the sample, this Chicken Rap1 GTPase- activating protein 2, RAP1GAP2 ELISA KIT includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus Chicken RAP1GAP2 concentration. The concentration of Chicken RAP1GAP2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
REAGENTS PROVIDED
All reagents provided are stored at 2-8 degree. Refer to the expiration date on the label.
MICROTITER PLATE 12x8strips
STANDARD 6x0.3ml vial
SAMPLE DILUENT 6.0ml
ENZYME CONJUGATE 10.0ml
WASH SOLUTION 25ml
SUBSTRATE A 6.0ml
SUBSTRATE B 6.0ml
STOP SOLUTION 6.0ml
Closure plate membrane 2
User manual 1
Sealed bags 1
Note:
1.This package insert must be read in its entirety before using this product.
2.This ELISA Kit is used for lab research only!
"(本产品仅供体外科研用途，不可用于临床诊断!)
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文献和实验Fluorescence Microscopy Study of Rap1 Subcellular Localization
The Ras-related GTPase Rap has been implicated in multiple cellular functions. In particular, Rap1 is a crucial regulator of both inside-out integrin activation and outside-in E-cadherin-mediated signaling. Thus, Rap1
with 250 µl Pierce Coomassie protein assay reagent in 96-well ELISA dishes and reading at 650 nm on a plate reader).The foregoing affinity purification steps were carried out at 4ºC.The proteins were dialyzed against PBS and stored at –20ºC.Protein
结合结构域（Raf RBD）与GFP的突变种YFP和CFP进行融合构建。他们将Ras和YFP融合，RafRBD与CFP融合，当两分子靠的足够近时，它们之间就会激发FRET，设计的蛋白Raichu-Ras， Raichu代表和Ras结合的嵌合单元。当把Raichu-Ras与特异性的GEFs（guanine-nucleotide exchange factors）和GAPs（GTPase-activating proteins）共表达，清楚的显示FRET的增加和减少与Ras突变体的激活和抑制
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