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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
见产品外包装
- 英文名：
BCIP/NBT Alkaline Phosphatase Substrate Kit For IHC
- 库存：
现货供应
- 供应商：
上海经科化学科技有限公司
- CAS号：
N/A
- 规格：
1kit
名称：BCIP/NBT Alkaline Phosphatase Substrate Kit For IHC BCIP/NBT显色试剂盒蓝紫色(IHC)
CAS：N/A
产品说明：本产品常应用于免疫学等相关实验中.
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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
salt NBT, (Altman, 1972) can be used in conjunction with X-Phos as the final electron acceptor for the indoxyl dimerization reaction. When reduced, NBT forms a purple precipitate. Of the cloned phosphatases, the human placental alkaline
Human Alkaline Phosphatase staining of transgenic embryo/tissue
. Wash in AP Buffer for 10 minutes. Stain with BM Purple AP Substrate at 4oC for 0.5 to 36 hours (N.B. Incubation at RT will give a quicker result but may result in seepage of staining. Also: NBT/BCIP AP staining has been tried with 9.5dpc embryos
Human Alkaline Phosphatase staining of transgenic embryo/tissue
phosphatase activity by incubation in PBS at 70-75o C for 30 minutes. Rinse in PBS at RT. Wash in AP Buffer for 10 minutes. Stain with BM Purple AP Substrate at 4o C for 0.5 to 36 hours (N.B. Incubation at RT will give a quicker result
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