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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
见产品外包装
- 英文名:
General Protease Inhibitor Cocktail,EDTA-free,100× DMSO Stock Solution
- 库存:
现货供应
- 供应商:
上海经科化学科技有限公司
- CAS号:
N/A
- 规格:
1ml
名称:General Protease Inhibitor Cocktail,EDTA-free,100× DMSO Stock Solution 广谱型蛋白酶抑制剂混合物(不含EDTA,100× DMSO储液)
CAS:N/A
产品说明:本产品常应用于蛋白研究等相关实验中.
产品详情:请咨询 <上海经科化学科技有限公司-客服>
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文献和实验Partial proteolysis with Staph. aureus V8 protease
. If you have too much, the V8 won't work. The molarity of neat mercaptoethanol is approximately 14. 1 microliter of ME per 14 ml of solution gives a 1 mmolar solution. Enzyme stock solution: 500 micrograms/ml (or 500 nanograms/microliter) V8-protease in 125
. Cocktail solutions are stored at -20 degrees and are good for approximately 6 months. I. Protease Inhibitor Cocktail The Protease inhibitor cocktail is used as 100X for extract preps and as 1000X for column elution buffers**. Cocktail
General Principles of Immunoprecipitation
.We usually use 2 mmolar.Sodium vanadate inhibits all tyrosine protein phosphatases.We use 200 micromolar and add it fresh for each experiment from a stock made in water and stored in a plastic tube at room temperature.Sodim fluoride is an inhibitor of Serine
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