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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
见产品外包装
- 英文名:
Alkaline Phosphatase(Powder)from Calf Intestine,Conjugation Grade,3000-6000 U/mg protein
- 库存:
现货供应
- 供应商:
上海经科化学科技有限公司
- CAS号:
9001-78-9
- 规格:
10MG
名称:Alkaline Phosphatase(Powder)from Calf Intestine,Conjugation Grade,3000-6000 U/mg protein 小牛肠碱性磷酸酶(冻干粉)
CAS: 9001-78-9
产品说明:本产品常应用于蛋白研究等相关实验中.
产品详情:请咨询 <上海经科化学科技有限公司-客服>
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文献和实验Mix a 5-µL aliquot of the phosphopeptides affinity-bound to the IMAC media (from Step 10) with 5 µL of 50 mM NH4HCO3 (pH 7.8) and 1 unit of calf intestine alkaline phosphatase in a 0.7-mL microcentrifuge tube. Incubate the tube on a slowly rotating
Protocol for 5' End Labeling RNA
10X Dephosphorylation Buffer (0.5 M Tris, pH 8.5, 1 mM EDTA, pH 8) 1 µl Calf Intestinal Phosphatase (CIP; 0.1 U/µl) 2) Incubate 1 hr at 37°C. Remove the Calf Intestine Alkaline Phosphatase by use of the Phosphatase Removal Reagent
with 20 ng of calf intestine alkaline phosphatase treated linear double stranded pUC cloning vector (Pharmacia), although the exact amount may vary depending on the yield of amplified DNA from the PCR reaction. 1.The reaction can be cleaned using 1 μl
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