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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
见产品外包装
- 英文名:
EHA105(pSoup)Electrocompetent Cell
- 库存:
现货供应
- 供应商:
上海经科化学科技有限公司
- CAS号:
N/A
- 规格:
10×50μl/50×50μl
| 规格: | 10×50μl | 产品价格: | ¥600.0 |
|---|---|---|---|
| 规格: | 50×50μl | 产品价格: | ¥2400.0 |
名称:EHA105(pSoup)Electrocompetent Cell 农杆菌电转感受态细胞
CAS:N/A
产品说明:本产品常应用于分子生物学等相关实验中.
产品详情:请咨询 <上海经科化学科技有限公司-客服>
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文献和实验Preparation of Electrocompetent Cells and Electroporation of Plasmid DNA
Preparation of Electrocompetent Cells and Electroporation of Plasmid DNA Inoculate 5-ml L-broth with a single colony of E. coli . Incubate 5 hours to overnight at 37°C on a roller or with moderate shaking.
Transduction Protocol for Adherent Cell Types
- The starting number of cells may be varied to account for total cell availability. Successful transductions have been carried with as little as 2.0 x 105 cells per well. - The duration the viral supernatant is on the cells can go from 4 hours to 2 days
should be at least 105 cells per milliliter. Common errors occur by improper mixing of the cell suspension prior to sampling and/or by allowing the cells to settle in the pipet prior to loading the hemocytometer counting chamber. Avoid the counting of multiple cell
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