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- 英文名:
K599 Electrocompetent Cell
- 库存:
现货供应
- 供应商:
上海经科化学科技有限公司
- CAS号:
N/A
- 规格:
10×50μl/50×50μl
| 规格: | 10×50μl | 产品价格: | ¥720.0 |
|---|---|---|---|
| 规格: | 50×50μl | 产品价格: | ¥3000.0 |
名称:K599 Electrocompetent Cell 农杆菌电转感受态细胞
CAS:N/A
产品说明:本产品常应用于分子生物学等相关实验中.
产品详情:请咨询 <上海经科化学科技有限公司-客服>
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文献和实验Preparation of Electrocompetent Cells and Electroporation of Plasmid DNA
Preparation of Electrocompetent Cells and Electroporation of Plasmid DNA Inoculate 5-ml L-broth with a single colony of E. coli . Incubate 5 hours to overnight at 37°C on a roller or with moderate shaking.
Recombineering‐Based Procedure for Creating BAC Transgene Constructs for Animals and Cell Lines
. The recombineering plasmid, pKD46, is used to fabricate BAC transgenic constructs that can be used in generating transgenic organisms as well as used in mammalian cell culture. Curr. Protoc. Mol. Biol. 95:23.14.1?23.14.28. © 2011 by John Wiley & Sons
and cTc inducible) were grown overnight in a shaker (Eppendorf) at 30°C, 1100 rpm. Next day electrocompetent cells were made from 30 ml cultures following the general manual of GeneBridges. At a cell density of OD 0.2, L -arabinose was added to the medium
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