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- 英文名:
C58C1 Chemically Competent Cell
- 库存:
现货供应
- 供应商:
上海经科化学科技有限公司
- CAS号:
N/A
- 规格:
10×100μl/50×100μl
| 规格: | 10×100μl | 产品价格: | ¥430.0 |
|---|---|---|---|
| 规格: | 50×100μl | 产品价格: | ¥1830.0 |
名称:C58C1 Chemically Competent Cell 农杆菌化学感受态细胞
CAS:N/A
产品说明:本产品常应用于分子生物学等相关实验中.
产品详情:请咨询 <上海经科化学科技有限公司-客服>
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文献和实验Preparing chemically competent cells
for 10 min. All subsequent steps should be carried out at 4o C and the cells should be kept on ice wherever possible Centrifuge for 10 minutes at 3000 rpm and 4o C. Remove supernatant. The cell pellets should be sufficiently solid
Transforming chemically competent cells
back into a larger volume can also help. Let sit for 30 minutes on ice. Note: If you are in a rush, you can shorten this incubation time to 5-10 min. Incubate cells for 30 seconds at 42o C. Note
TOP10 chemically competent cells
Detergent is a major inhibitor of competent cell growth and transformation. Glass and plastic must be detergent free for these protocols. The easiest way to do this is to avoid washing glassware, and simply rinse it out. Autoclaving glassware filled
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