- ￥3505.95
- Lucigen
- http://lucigen.com/store/docs/manuals/MA133-Endura-Competent-Cells.pdf
- 60240-2
- 2026年04月08日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件：
-80º C
- 英文名：
Endura™ Chemically Competent Cells
- 库存：
大量
- 供应商：
中北林格
- 规格：
24 rxns (DUOs)
Endura™ Competent Cells
产品说明书
- Clone unstable sequences with reliable results
- Direct replacement for Invitrogen Stbl3™ and Agilent SURE strain
- Available as chemically competent or electrocompetent cells
- Your choice of two packaging formats
- High efficiency: over 1 × 107 cfu/µg (chem) or 1 × 1010 cfu/µg (electro)
- Ideal for CRISPR GeCKO Libraries
- Excellent value
Conserve clones that contain unstable sequences!
Maintain unstable DNA. Lucigen’s Endura Competent Cells are a commonly used strain for cloning sequences that suffer unwanted recombination events in other strains. Clones with inverted repeats or other sequences prone to recombination are commonly found in retroviral genes, and require cells such as Endura to be propagated stably.
Excellent value and efficiency! Switch to Endura cells today and experience a higher level of efficiency and a savings in your cloning budget.
Chemical or Electrocompetent. Whichever method of transformation you prefer, Lucigen can give you better efficiency and/or better prices.
Figure 1. Cost per reaction of chemically competent cells of clone-stabilizing strains from other suppliers.
Figure 2. Transformation efficiencies of electrocompetent clone-stabilizing strains.
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Ordering
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Specifications
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Manuals
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Resources
Endura™ Competent Cells include Control DNA and Recovery Medium, and are packaged as SOLOs (1 transformation per tube) or DUOs (2 transformations per tube) as indicated. Endura Electrocompetent cells are provided in DUOs format only. Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.
PLEASE NOTE: Bulk quantities (10 times larger than the largest retail package size below) and/or custom packaging are available at very attractive prices for all Lucigen Competent Cells.
NOTE: Transformation of Endura Electrocompetent Cells with GeCKO lentiviral libraries may require extra Recovery Medium. The Zhang lab protocol (available from Addgene) recommends ~2 mL of Recovery Medium per transformation. Lucigen Endura kits provide 1 mL of Recovery Medium per transformation.
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文献和实验Transforming chemically competent cells
Method Thaw TSS cells on ice. Add DNA, pipette gently to mix (1μl of prepped plasmid is more than enough). Note: If you are adding small volumes (~1μl), be careful to mix the culture well. Diluting the plasmid
Preparing chemically competent cells
Materials Plate of cells to be made competent TSS buffer LB media Ice Glassware & Equipment Falcon tubes 500μl Eppendorf tubes, on ice 200ml conical flask 200μl pipetman or repeating
TOP10 chemically competent cells
may not be necessary Place in -80°C freezer indefinitely. Preparing competent cells Inoculate 250 ml of SOB medium with 1 ml vial of seed stock and grow at 20°C to an OD600nm of 0.3 This takes approximately 16 hours.
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