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- 英文名:
F-DH5a Chemically Competent Cell
- 库存:
现货供应
- 供应商:
上海经科化学科技有限公司
- CAS号:
N/A
- 规格:
20×100μl/100×100μl
| 规格: | 20×100μl | 产品价格: | ¥280.0 |
|---|---|---|---|
| 规格: | 100×100μl | 产品价格: | ¥1280.0 |
名称:F-DH5a Chemically Competent Cell 大肠杆菌化学感受态细胞
CAS:N/A
产品说明:本产品常应用于分子生物学等相关实验中.
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文献和实验实验概要 Competent cells are those that possess more easily altered cell walls that DNA can be passed through easily. These cells readily incorporate foreign DNA. On example of a competent cell is E. coli.
CCMB buffer to make chemical competent cell
TOP10 chemically competent cells ( modified from http://openwetware.org/wiki/TOP10_chemically_competent_cells#CCMB80_buffer) I also use this protocol to make Ecoli compent cell. It is pretty good. You can start from the fresh clone on plate
. Step 6: Centrifuge the cell suspension at 4,000 RPM in the GSA rotor for 10 minutes and resuspend the cell pellet in 1/50 volume of ice cold, sterile 85 mM CaCl2 in 15% glycerol w/v. Dispense in 100 mL aliquots and freeze cells at -80oC.
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