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- 文献和实验
- 技术资料
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- 英文名:
L-Asparagine
- 库存:
现货供应
- 供应商:
上海经科化学科技有限公司
- CAS号:
70-47-3
- 规格:
25g/100g
| 规格: | 25g | 产品价格: | ¥60.0 |
|---|---|---|---|
| 规格: | 100g | 产品价格: | ¥200.0 |
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文献和实验Permeabilization of gram-negative bacteria with KPi/hexane for the release of L-asparaginase
utilizes the determination of ammonia liberated from L-asparagine in the enzyme reaction by the Nessler reaction. L-asparaginase catalyzes the hydrolysis of L-asparagine to L-aspartic acid and ammonia. Start the reaction by adding 0.1 ml
Extraction of DNA from Schizophyllum commune (裂褶菌DNA提取)
and Thompson. Nucleic Acids Res. 8:4321-4325; 1980.) 0.1 P agar For 1 L total medium: 5 ml Phosphate stock 1 ml trace elements stock 5 ml thiamine HCl 1 g L-asparagine 0.5 g MgSO4 20 g Glucose 10 g Agar 0.002 P liquid medium For 1 L total medium
Permeabilization of gram-negative bacteria with KPi/hexane for the release of L-asparaginase
. Prepare cell suspensions also in distilled water or 0.05 M KPi with no hexane as controls. Measure the enzyme activity utilizing the Nesslerization reaction. This method utilizes the determination of ammonia liberated from L-asparagine
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