- ￥3280
- LSM BIO
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELA-E0355r
- 2025年11月07日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
大鼠
- 标记物：
Rat TG
- 样本：
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rat TG ELISA Kit
Product Name:Rat TG ELISA Kit
Packing:96T
Catalog No.:ELA-E0355r
Gene Name:TG
Detect Range:15.6-1000 pg/mL
Sensitivity:6.1 pg/mL
Target Protein Name:TG
Alternative Name:TG
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Rat TG ELISA Kit allows for the in vitro quantitative determination of TG concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human LRP2 ELISA Kit has been pre-coated with an LRP2 antibody specific to Human Low-density lipoprotein receptor-related protein 2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Low-density lipoprotein receptor-related protein 2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Low-density lipoprotein receptor-related protein 2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human Low-density lipoprotein receptor-related protein 2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
充分摇匀后，室温放置5-10分钟，3500转/分钟，吸弃上清液，测各沉淀管的放射性计数（p）。 四．结果分析 1．参考值：TG抗体B/T≥30%为阳性。 2．临床意义：可用于自身免疫疾病的诊断。 五注意事项 1收到试剂盒后，尽快存放在2-8°C冰箱中。 2阴性对照管在数据处理时非常重要，要做复管。 3分离剂加前一定要摇匀。 4加样时应避免血清样品间的交叉污染。 5加样前试剂要平衡到室温并摇匀。 六质量控制 1每批测定都使用北京
at 4°C for 15 minutes. 9. Remove and aliquot supernatant. We recommend making several 50 μl aliquots. 10. Before running ELISA, dilute protein 1:100 and run a Bradford total protein assay or Quant-iT™ Protein Quantitation Kit assay (Cat. no. Q
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