Established from the poorly differentiated esophageal squamous cell carcinoma resected from upper (cervical) esophagus of a 49-year-old Japanese woman after receiving radiotherapy (tumor was invading contiguous structures); described as carrying amplified oncogenes, c-erb-B (8x) and cyclin D1 (4x) and producing tumors in nude mice.
种属
人
年龄(性别)
女;49岁
组织来源
食管
生长特性
贴壁细胞
细胞形态
上皮细胞样
细胞类型
肿瘤细胞
肿瘤类型
食管癌细胞
生物安全等级
1
生长培养基
RPMI-1640+Ham's F-12+10% FBS+1%P/S
推荐传代比例
1:4-1:8
推荐换液频率
2~3次/周
倍增时间
13.7 hours (PubMed=1728357); 23 hours (PubMed=25984343); <25 hours, less when serum amount is increased (DSMZ)