The cells were transformed by infection with the NTKmT retrovirus vector that expresses polyomavirus middle T antigen
种属
小鼠
年龄(性别)
6周龄
组织来源
大脑皮层
生长特性
贴壁细胞
细胞形态
上皮细胞样
细胞类型
转化细胞系
生物安全等级
1
生长培养基
DMEM+10% FBS+1% P/S
推荐传代比例
1:4-1:6
推荐换液频率
2~3次/周
倍增时间
~24-32 hours
冻存条件
冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮
培养条件
气相:空气,95%;CO2,5% 温度:37℃
抗原表达情况
ICAM-1 +; VCAM-1 +; MAdCAM-1 +
基因表达情况
von Willebrand factor,ICAM-1 +; VCAM-1 +; MAdCAM-1 +,The endothelial nature of these cells was confirmed by the observed expression of von Willebrand factor and uptake of fluorescently labeled low density lipoprotein (LDL).,The expression of Peyer's Patch high endothelial receptor for lymphocytes, the mucosal vascular addressin (MAdCAM-1) and E-selectin can be induced on bEnd