- ￥2980
- LSM Bio
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELA-E2110r
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
大鼠
- 标记物：
Rat Adenosine triphosphate,ATP
- 样本：
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rat Adenosine triphosphate, ATP ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Adenosine triphosphate, Adenosine-5'-triphosphate , ATP
Search name
Rat Adenosine triphosphate ELISA Kit, Rat ATP ELISA Kit, Rat Adenosine-5'-triphosphate ELISA Kit
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat Adenosine triphosphate concentrations in serum, plasma,
tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to Adenosine triphosphate, During the reaction, Adenosine triphosphate in the sample or standard
competes with a fixed amount of biotin-labeled Adenosine triphosphate for sites on a pre-coated Monoclonal antibody specific to
Adenosine triphosphate. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to
Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The
enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Adenosine triphosphate in the samples is then determined
by comparing the O.D. of the samples to the standard curve.
PDF manual download
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文献和实验腹苷三磷酸的简称。由腺嘌吟、核糖和 3个磷酸基团连接而成，水解时释放出能量较多，是生物体内最直接的能量来源。
Extraction and Quantification of Adenosine Triphosphate in Mammalian Tissues and Cells
Adenosine 5′-triphosphate (ATP) is the “energy currency” of organisms and plays central roles in bioenergetics, whereby its level is used to evaluate cell viability, proliferation, death, and energy transmission. In this chapter
简称ATP （俄文 AT Φ）。最早发现的是具有高能磷酸键的一种核苷酸（ C.H.Fiske ， Y.Subbarow ， 1929 ； K.Lohmann ， 1929 ），最初认为只是糖酵解或发酵的磷酸供体，但后来明确了它是作为代表性的能量传递体参与许多生化反应，在能量获得和利用上起着重要作用。其末端焦磷酸键的分解： ATP H 2 O → ADP H 3 PO 4 的标准自由能变化，在 pH 中性的水溶液里约为 7.3 千卡 / 摩耳。广泛分布于生物体内。例如，哺乳类的骨骼
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