- ￥2980
- LSM Bio
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELA-E0162r
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
大鼠
- 标记物：
Rat Glutamine synthetase,GS
- 样本：
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rat Glutamine synthetase, GS ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Glutamine synthetase,GS,Glutamate decarboxylase,Glutamate--ammonia ligase,Glutamine synthetase,GLNS; PIG43; PIG59; cell proliferation-inducing protein 59; glutamate decarboxylase; glutamate--ammonia ligase; glutamine synthase; proliferation-inducing protein 43; glutamate-ammonia ligase,GLUL
Search name
Rat Glutamine synthetase ELISA KIT ,Rat GS ELISA KIT ,Rat GLUL ELISA KIT, Rat GLNS ELISA KIT ,Rat PIG43 ELISA KIT ,Rat PIG59 ELISA KIT ,Rat cell proliferation-inducing protein 59 ELISA KIT ,Rat glutamate decarboxylase ELISA KIT ,Rat glutamate--ammonia ligase ELISA KIT,Rat Glutamate decarboxylase ELISA KIT ,Rat Glutamate--ammonia ligase ELISA KIT ,Rat Glutamine synthetase ELISA KIT ,Rat GLNS ELISA KIT ,Rat PIG43 ELISA KIT ,Rat PIG59 ELISA KIT ,Rat cell proliferation-inducing protein 59 ELISA KIT ,Rat glutamate decarboxylase ELISA KIT ,Rat glutamate--ammonia ligase ELISA KIT ,Rat glutamine synthase ELISA KIT ,Rat proliferation-inducing protein 43 ELISA KIT ,Rat glutamate-ammonia ligase ELISA KIT,Rat GLUL ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat glutamine synthetase,GS concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to GS. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for GS and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain GS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GS in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验缩写为 GS。为在 ATP的存在下使氨与谷氨酸结合生成谷氨酰胺的酶， EC． 6． 3． 1． 2． L-谷氨酸 氨 ATPL- 谷氨酰胺 ADP Pi反应机制很复杂，要经过酶蛋白与γ -谷氨酰磷酸的中间体。与谷氨酰胺合成反应不同的是可将γ -谷氨酰基转移于适当的受体，具有如羟胺的γ -谷氨酰基转移酶的活性。广泛分布于微生物和高等动植物中，除了可供给谷氨酰氨外，还有与谷氨酸合成酶偶联向α -氨基酸供给氨基的重要功能。大肠杆菌和枯草菌的酶是由分子量为 5万的单一的 12个亚基
Preparation of Isolated Periportal or Perivenous Hepatocytes from Rat Liver
(6 ), whereas enzymes such as glucokinase (7 ), P-450-dependent hydroxyl-ation reactions (8 ), and glutamine synthetase (9 ) show the opposite zona-tion. Recent studies on lipogenic enzymes (acetyl-CoA carboxylase, citrate lyase, and fatty acid synthase
Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
Although Y3/Ag1.2.3 has its own myeloma light chain, their are numerous characteristics that make this a better parent line for making hybridomas than the 'Y0' chain loss variants or using NS0 to make rat x mouse hybrids. These are: a) Hybridomas
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