- ￥2980
- LSM Bio
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELI-43647r
- 2025年06月26日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
大鼠
- 标记物：
Rat Phosphatidylinositol-glycan-specific phospholipase D,Gpld
- 样本：
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rat Phosphatidylinositol- glycan- specific phospholipase D, GPLD1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Phosphatidylinositol-glycan-specific phospholipase D,PI-G PLD,Glycoprotein phospholipase D,Glycosyl-phosphatidylinositol-specific phospholipase D,GPI-PLD,GPI-specific phospholipase ,GPLD1,GPIPLD; GPIPLDM; PIGPLD; PIGPLD1; PLD; GPI-PLD; GPI-specific phospholipase D; PI-G PLD; glycoprotein phospholipase D; phosphatidylinositol-glycan-specific phospholipase D; glycosylphosphatidylinositol specific phospholipase D1
Search name
Rat Phosphatidylinositol-glycan-specific phospholipase D ELISA KIT ,Rat PI-G PLD ELISA KIT ,Rat Glycoprotein phospholipase D ELISA KIT ,Rat Glycosyl-phosphatidylinositol-specific phospholipase D ELISA KIT ,Rat GPI-PLD ELISA KIT ,Rat GPI-specific phospholipase ELISA KIT ,Rat GPLD1 ELISA KIT ,Rat GPIPLD ELISA KIT ,Rat GPIPLDM ELISA KIT ,Rat PIGPLD ELISA KIT ,Rat PIGPLD1 ELISA KIT ,Rat PLD ELISA KIT ,Rat GPI-PLD ELISA KIT ,Rat GPI-specific phospholipase D ELISA KIT ,Rat PI-G PLD ELISA KIT ,Rat glycoprotein phospholipase D ELISA KIT ,Rat phosphatidylinositol-glycan-specific phospholipase D ELISA KIT ,Rat glycosylphosphatidylinositol specific phospholipase D1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat phosphatidylinositol-glycan-specific phospholipase d,PI-G PLD concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to PI-G PLD. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for PI-G PLD and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain PI-G PLD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PI-G PLD in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Phosphatidylinositol 4-Kinase Assay in Ovarian Carcinoma Cells
. Metabolic pathways including PI 4-kinase are now known as classical PI turnover pathways. PI-4-P formed with PI 4-kinase is then phosphorylated by PI-4-P kinase into PI-4,5-P2 . Phosphatidylinositol-specific phospholipase C(PI-PLC) is the rate-limiting
sialic acid in different rat organs, using N‐propanoyl‐D‐hexosamines as precursors. J. Biol. Chem. 267:16934‐16938. Keppler, O.T., Horstkorte, R., Pawlita, M., Schmidt, C
from the cell membrane by the action of a specific bacterial phospholipase, phosphatidylinositol phospholipase C (PI-PLC) (5 ). The PI-PLC cleaves the anchor at the phosphodiester bond between the phophinositol group and the lipid portion of the anchor (Fig
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