- ￥2980
- LSM Bio
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELI-37426r
- 2025年07月07日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
大鼠
- 标记物：
Rat Period circadian protein homolog 1,Per1
- 样本：
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rat Period circadian protein homolog 1, PER1 ELISA Kit(CUSTOM)
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
PER1,PER; RIGUI; hPER; Period, drosophila, homolog of; circadian clock protein PERIOD 1; circadian pacemaker protein RIGUI; hPER1; period circadian protein homolog 1; period homolog 1; period circadian clock 1
Search name
Rat PER1 ELISA KIT ,Rat PER ELISA KIT ,Rat RIGUI ELISA KIT ,Rat hPER ELISA KIT ,Rat Period drosophila homolog of ELISA KIT ,Rat circadian clock protein PERIOD 1 ELISA KIT ,Rat circadian pacemaker protein RIGUI ELISA KIT ,Rat hPER1 ELISA KIT ,Rat period circadian protein homolog 1 ELISA KIT ,Rat period homolog 1 ELISA KIT ,Rat period circadian clock 1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat Period circadian protein homolog 1, PER1 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique.The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat Period circadian protein homolog 1, PER1, During the reaction, Rat Period circadian protein homolog 1, PER1 in the sample or standard competes with a fixed amount of biotin-labeled Rat Period circadian protein homolog 1, PER1 for sites on a pre-coated Monoclonal antibody specific to Rat Period circadian protein homolog 1, PER1. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat Period circadian protein homolog 1, PER1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Customized product Min.Order 5kits
Delivery time 4-6month
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文献和实验Enzyme-Linked ImmunoSorbent Assay (ELISA) for NGF
as described previously, but do not leave dried wells for an extended period of time.• SAMPLE PREPARATIONA volume of 400 µl per sample is the minimum amount needed to obtain duplicate values (100 µl per well for 2 GIG and 2 GAM wells). 0.1% Tween is added
V294.PART II，Chapter 5 细胞粘附实验（英文版）
atmospherefor a prolonged period of time (e.g., 2 h), the wells are examined under amicroscopy and the number of cells that form monolayer reaching 100%confluence represents the maximal number of cells that can adhere to the coatedextracellular matrix protein
, Bcl 2 and P53 protein xpressions in tissues from hepatocellular carcinoma. Huaren Xiaohua Zazhi,1998;6:834-836 [35] Wang JM, Zou Q, Zou SQ. The role of bcl-2 gene in apoptosis of li ver in rat with obstructive jaundice.Shijie Huaren Xiaohua Zazhi,1999
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