Rat Submandibular gland secret

Rat Submandibular gland secretory Glx- rich protein CA, Grpca ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Grpca,Grp-Ca; Grpca_m; glutamine/glutamic acid-rich protein A, mapped; glutamine/glutamic acid-rich protein GRP-Ca; submandibular gland secretory Glx-rich protein CA; glutamine/glutamic acid-rich protein A

Search name

Rat Grpca ELISA KIT ,Rat Grp-Ca ELISA KIT ,Rat Grpca_m ELISA KIT ,Rat glutamine/glutamic acid-rich protein A mapped ELISA KIT ,Rat glutamine/glutamic acid-rich protein GRP-Ca ELISA KIT ,Rat submandibular gland secretory Glx-rich protein CA ELISA KIT ,Rat glutamine/glutamic acid-rich protein A ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Rat Submandibular gland secretory Glx- rich protein CA, Grpca concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Grpca. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Grpca and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Grpca, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Grpca in the samples is then determined by comparing the O.D. of the samples to the standard curve.