- ￥2980
- LSM Bio
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELI-40864r
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
大鼠
- 标记物：
Rat Secretory carrier-associated membrane protein 1,Scamp1 ELI
- 样本：
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rat Secretory carrier- associated membrane protein 1, SCAMP1 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Secretory carrier-associated membrane protein 1,Secretory carrier membrane protein 1, SCAMP1,SCAMP; SCAMP37; secretory carrier-associated membrane protein 1; secretory carrier membrane protein 1
Search name
Rat Secretory carrier-associated membrane protein 1 ELISA KIT ,Rat Secretory carrier membrane protein 1 ELISA KIT ,Rat SCAMP1 ELISA KIT ,Rat SCAMP ELISA KIT ,Rat SCAMP37 ELISA KIT ,Rat secretory carrier-associated membrane protein 1 ELISA KIT ,Rat secretory carrier membrane protein 1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat secretory carrier-associated membrane protein 1,SCAM1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to SCAM1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for SCAM1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain SCAM1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of SCAM1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验and native two?dimensional PAGE. Finally, the removal of Coomassie dye from electroeluted proteins is detailed along with a discussion of fundamental considerations for the solubilization of membrane protein complexes from various biological samples
in the range of 5 to 8.5. Under the influence of the electrical force the pH gradient will be established by the carrier ampholytes, and the protein species migrate and focus (concentrate) at their isoelectric points. The focusing effect of the electrical force
.5.Under the influence of the electrical force the pH gradient will be established by the carrier ampholytes, and the protein species migrate and focus (concentrate) at their isoelectric points. The focusing effect of the electrical force is counteracted by diffusion
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