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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法
- 应用:
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种:
大鼠
- 标记物:
Rat Carboxypeptidase E,Cpe
- 样本:
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
46.9pg/ml
- 规格:
96tests
Rat Carboxypeptidase E, CPE ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
CPE,carboxypeptidase E; CPH; carboxypeptidase H; cobalt-stimulated chromaffin granule carboxypeptidase; enkephalin convertase; insulin granule-associated carboxypeptidase; prohormone-processing carboxypeptidase
Search name
Rat CPE ELISA KIT ,Rat carboxypeptidase E ELISA KIT ,Rat CPH ELISA KIT ,Rat carboxypeptidase H ELISA KIT ,Rat cobalt-stimulated chromaffin granule carboxypeptidase ELISA KIT ,Rat enkephalin convertase ELISA KIT ,Rat insulin granule-associated carboxypeptidase ELISA KIT ,Rat prohormone-processing carboxypeptidase ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat Carboxypeptidase E, CPE concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to CPE. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for CPE and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain CPE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CPE in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验E L I S A w i t h p l a t e l e t s
OUTLINE This modification of qualitative ELISA (Enzyme-Linked Immunosorbent Assay) is used for either screening detection of anti-platelet antibodies or for detection of platelet-associated Ig (PAIg) (shown here). PROTOCOL
cathy12303 有没有哪位前辈用过cAMP的ELISA试剂盒? dianaofyezi 我 有问题可以站内消息或者QQ52187644联系。 白大褂2009 你好,我是新手,你用过的ELISA试剂在哪买的,哪个公司的比较好啊?还有,测定大鼠脑组织里的cAMP,放免法和ELISA法各有什么优缺点啊?谢谢回复!! songdianwei 我在 R&D公司买的,测
Visualization of Peptide Secretory Vesicles in Living Nerve Cells
of overexpression of exogenous peptides comprising of the cytoplasmic tail sequence of vesicular carboxypeptidase E (CPE), proposed to be involved in the mechanism of trafficking of peptidergic secretory vesicles, in live hippocampal neurons
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