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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法
- 应用:
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种:
大鼠
- 标记物:
Rat Keratin type I cytoskeletal 20,KRT20
- 样本:
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
46.9pg/ml
- 规格:
96tests
Rat Keratin type I cytoskeletal 20, KRT20 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Keratin, type I cytoskeletal 20,Protein IT,Cytokeratin-20,Keratin-20,KRT20,CK20; CD20; CK-20; K20; KRT21; cytokeratin 20; cytokeratin-20; keratin, type I cytoskeletal 20; keratin-20; protein IT; keratin 20
Search name
Rat Keratin ELISA KIT ,Rat type I cytoskeletal 20 ELISA KIT ,Rat Protein IT ELISA KIT ,Rat Cytokeratin-20 ELISA KIT ,Rat Keratin-20 ELISA KIT ,Rat KRT20 ELISA KIT ,Rat CK20 ELISA KIT ,Rat CD20 ELISA KIT ,Rat CK-20 ELISA KIT ,Rat K20 ELISA KIT ,Rat KRT21 ELISA KIT ,Rat cytokeratin 20 ELISA KIT ,Rat cytokeratin-20 ELISA KIT ,Rat keratin type I cytoskeletal 20 ELISA KIT ,Rat keratin-20 ELISA KIT ,Rat protein IT keratin 20 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat keratin, type i cytoskeletal 20,Keratin, type I cytoskeletal 20 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Keratin, type I cytoskeletal 20. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Keratin, type I cytoskeletal 20 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Keratin, type I cytoskeletal 20, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Keratin, type I cytoskeletal 20 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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