Rat Peroxisome proliferator- a

Rat Peroxisome proliferator- activated receptor gamma coactivator 1- alpha, PPARGC1A ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Peroxisome proliferator-activated receptor gamma coactivator 1-alpha,PGC-1-alpha,PPAR-gamma coactivator 1-alpha,PPARGC-1-alpha,Ligand effect modulator 6, PPARGC1A,LEM6; PGC-1(alpha); PGC-1v; PGC1; PGC1A; PPARGC1; L-PGC-1alpha; PGC-1-alpha; PPAR gamma coactivator variant form; PPARGC-1-alpha; ligand effect modulator-6; peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B4; peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B4-3ext; peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B4-8a; peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B5; peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B5-NT; peroxisome proliferator-activated receptor gamma coactivator 1-alpha; peroxisome proliferator-activated receptor gamma coactivator 1 alpha

Search name

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Intended use

This immunoassay kit allows for the in vitro quantitative determination of rat peroxisome proliferator-activated receptor gamma coactivator 1-alpha,PGC-1-alpha concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to PGC-1-alpha. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for PGC-1-alpha and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain PGC-1-alpha, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PGC-1-alpha in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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