- ￥2980
- LSM Bio
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELI-12281r
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
大鼠
- 标记物：
Rat 14-3-3 protein eta,Ywhah
- 样本：
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rat 14- 3- 3 protein eta, YWHAH ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Protein AS1,YWHAH,14 3 3 protein eta,YWHA1; 14-3-3 eta; 14-3-3 protein eta; tyrosine 3-monooxygenase activation protein, eta polypeptide,tryptophan 5-monooxygenase activation protein eta polypeptide
Search name
Rat Protein AS1 ELISA KIT ,Rat YWHAH ELISA KIT ,Rat 14 3 3 protein eta ELISA KIT ,Rat YWHA1 ELISA KIT ,Rat 14-3-3 eta ELISA KIT ,Rat 14-3-3 protein eta ELISA KIT ,Rat tyrosine 3-monooxygenase activation protein eta polypeptide ELISA KIT ,Rat tryptophan 5-monooxygenase activation protein eta polypeptide ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat 14- 3- 3 protein eta, YWHAH concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to YWHAH, During the reaction, YWHAH in the sample or standard competes with a fixed amount of biotin-labeled YWHAH for sites on a pre-coated Monoclonal antibody specific to YWHAH. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of YWHAH in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Customized Product
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文献和实验-3-3 protein and multiple signalling pathwaysChromosome assignment of human brain 14-3-3 protein eta chainSubcellular localisation of 14-3-3 isoforms in rat brain using specific antibodiesSite of interaction of 14-3-3 protein with phosphorylated tryptophan
Introduction of human 14-3-3 proteins
-3-3 protein and multiple signalling pathwaysChromosome assignment of human brain 14-3-3 protein eta chainSubcellular localisation of 14-3-3 isoforms in rat brain using specific antibodies---------------------------------------------------------
at 4°C for 15 minutes. 9. Remove and aliquot supernatant. We recommend making several 50 μl aliquots. 10. Before running ELISA, dilute protein 1:100 and run a Bradford total protein assay or Quant-iT™ Protein Quantitation Kit assay (Cat. no. Q
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