Rat ATP- binding cassette sub-

Rat ATP- binding cassette sub- family A member 7, ABCA7 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

ABCA7,ABCA-SSN; ABCX; ATP-binding cassette sub-family A member 7; autoantigen SS-N; macrophage ABC transporter, Autoantigen SS-N, ABC1 member 7

Search name

Rat ABCA7 ELISA KIT ,Rat ABCA-SSN ELISA KIT ,Rat ABCX ELISA KIT ,Rat ATP-binding cassette sub-family A member 7 ELISA KIT ,Rat autoantigen SS-N ELISA KIT ,Rat macrophage ABC transporter ELISA KIT ,Rat  Autoantigen SS-N ELISA KIT ,Rat ABC1 member 7 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of rat atp-binding cassette sub-family a member 7,ABCA7 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to ABCA7. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for ABCA7 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain ABCA7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ABCA7 in the samples is then determined by comparing the O.D. of the samples to the standard curve.