Rat Alpha- 1- macroglobulin, A

Rat Alpha- 1- macroglobulin, A1m ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Pzp,A1M,alpha 1 macroglobulin,alpha-1-macroglobulin 165 kDa subunit; alpha-1-macroglobulin; A1m; alpha-1-M; pregnancy-zone protein

Search name

Rat Pzp ELISA KIT ,Rat A1M ELISA KIT ,Rat alpha 1 macroglobulin ELISA KIT ,Rat alpha-1-macroglobulin 165 kDa subunit ELISA KIT ,Rat alpha-1-macroglobulin ELISA KIT ,Rat alpha-1-M ELISA KIT ,Rat pregnancy-zone protein ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Rat Alpha- 1- macroglobulin, A1m beta concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat Alpha- 1- macroglobulin, A1m. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Rat Alpha- 1- macroglobulin, A1m and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Rat Alpha- 1- macroglobulin, A1m, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat Alpha- 1- macroglobulin, A1m in the samples is then determined by comparing the O.D. of the samples to the standard curve.