Rat Alpha- 2- macroglobulin, A

Rat Alpha- 2- macroglobulin, A2M ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Alpha-2-macroglobulin,Alpha-2-M,C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5, alpha 2 macroglobulin,C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5,A2MD,CPAMD5,FWP007,S863-7,alpha-2-M

Search name

Rat Alpha-2-macroglobulin ELISA KIT ,Rat Alpha-2-M ELISA KIT ,Rat C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5 ELISA KIT ,Rat alpha 2 macroglobulin ELISA KIT ,Rat C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5 ELISA KIT ,Rat A2MD ELISA KIT ,Rat CPAMD5 ELISA KIT ,Rat FWP007 ELISA KIT ,Rat S863-7 ELISA KIT ,Rat alpha-2-M ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Rat alpha-2-macroglobulin,Alpha-2-M concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-2-M. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Alpha-2-M and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Alpha-2-M, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Alpha-2-M in the samples is then determined by comparing the O.D. of the samples to the standard curve.