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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法
- 应用:
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种:
大鼠
- 标记物:
Rat Apelin 36
- 样本:
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
46.9pg/ml
- 规格:
96tests
Rat Apelin- 36 , AP36 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!
PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Apelin-36, APLN,apelin,APEL; XNPEP2; AGTRL1 ligand; APJ endogenous ligand, APLN36
Search name
Rat Apelin-36 ELISA Kit,Apelin-36 ELISA Kit, APLN36 ELISA Kit, Rat APLN ELISA Kit
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat apelin-36,Apelin-36 concentrations in serum, Plasma,
Urine, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apelin-36. Standards or samples are then added
to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Apelin-36 and Avidin
conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to
each well. Only those wells that contain Apelin-36, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color.
The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Apelin-36 in the samples is then determined by comparing
the O.D. of the samples to the standard curve.
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文献和实验上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人Apelin (Apelin)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 Apelin 单抗包被于酶标板上,标准品和样品中的 Apelin与单抗结合,加入生物素化的抗人
人 Apelin 酶联免疫分析( ELISA ) 试剂盒使用说明书 本试剂仅供研究使用 目的:本试剂盒用于测定人血清,血浆及相关液体样本中Apelin 的 含量。 实验原理: 本试剂盒应用双抗体夹心法测定标本中人 Apelin 水平。用纯化的人 Apelin 抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入 Apelin ,再与 HRP 标记的 Apelin 抗体结合,形成抗体
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 大鼠 Apelin(Apelin)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗大鼠 Apelin 单抗包被于酶标板上,标准品和样品中的 Apelin 与单抗结合,加入生物素化的抗大鼠 Apelin ,形成免疫复合
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