- ￥2980
- LSM Bio
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELA-E1824r
- 2025年07月09日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
大鼠
- 标记物：
Rat Nuclear factor-kappa B,NF-魏B
- 样本：
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rat Nuclear factor-kappa B, NF-κB ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Nuclear factor-kappa B,NF-κB,Nuclear factor NF-kappa-B p105 subunit,Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1,DNA-binding factor KBF1,EBP-1,
Search name
Rat Nfkb ELISA KIT ,Rat Nuclear factor-kappa B ELISA KIT ,Rat NF-κB ELISA KIT ,Rat Nuclear factor NF-kappa-B p105 subunit ELISA KIT ,Rat Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 ELISA KIT ,Rat DNA-binding factor KBF1 ELISA KIT ,Rat EBP-1 ELISA KIT
Intended use
Rat Nfkb ELISA KIT allows for the in vitro quantitative determination of rat Nuclear factor-kappa B,NF-κB concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in Rat Nfkb ELISA KIT has been pre-coated with an antibody specific to NF-κB. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for NF-κB and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain NF-κB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of NF-κB in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Range 0.15-10ng/ml
Rat Nfkb ELISA KIT PDF Manual download
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文献和实验Activation of Nuclear Factor-B (NF-B) in Mononuclear Cells (MNC)
The transcription factor NF-κB was first identified as a regulator of the expression of the kappa light-chain gene in murine B lymphocytes (1 ). It has a central role in the immunological processes and regulates a wide range of genes
joan831 请教高手：ERK1/2与NF-kappa B两个信号通路关系如何？前者能激活后者吗？还有，若在文献中查到的某种细胞类型中（比如单核细胞）中ERK1/2与NF-kappa B两个信号通路的关系是否也适用于其他细胞类型（比如肾系膜细胞）？谢谢！ lijinbaobao 前者能激活后者，应该在其他的细胞类型中也适用。 lwjssry 一种细胞的信号通路除非你证明了也存在于另一
Activation of Nuclear Factor-B
Nuclear factor-κB (NF-κB) is a key transcription factor that regulates the expression of genes involved in immune and inflammatory responses and in cell death and survival. This chapter describes in detail the method for measuring the NF-κB
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