- ￥2980
- LSM Bio
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELI-06441r
- 2026年01月13日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
大鼠
- 标记物：
Rat Apolipoprotein B-100,Apob
- 样本：
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rat Apolipoprotein B- 100, APOB ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
ApoB,apolipoprotein B,apoB-48; apoB-100; FLDB; LDLCQ4; apo B-100; apolipoprotein B (including Ag(x) antigen); apolipoprotein B-100; apolipoprotein B48; mutant Apo B 100
Search name
Rat ApoB ELISA KIT ,Rat apolipoprotein B ELISA KIT ,Rat apoB-48 ELISA KIT ,Rat apoB-100 ELISA KIT ,Rat FLDB ELISA KIT ,Rat LDLCQ4 ELISA KIT ,Rat apo B-100 ELISA KIT ,Rat apolipoprotein B (including Ag(x) antigen) ELISA KIT ,Rat apolipoprotein B-100 ELISA KIT ,Rat apolipoprotein B48 ELISA KIT ,Rat mutant Apo B 100 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat apolipoprotein b-100,Apo B-100 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apo B-100. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Apo B-100 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Apo B-100, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Apo B-100 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF Manual download
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文献和实验Purification of Rat Hepatic Lipase Essentially Free of Apolipoprotein E and Apolipoprotein B
subfractions. In isolated, perfused rat livers inhibition of hepatic lipase with antibodies or removal of HL by heparin perfusion reduces the uptake of chylomicron remnants (13 ). Finally, recent studies with cultured hepatoma cells over-expressing human HL
人的载脂蛋白有两种表达形式: apoB-48和apoB-100。该基因的序列在肝、肠组织细胞中是相同的,但产物不同。在肝细胞中,产物为4563个氨基酸残基的肽：apoB-100; 但在肠细胞中,产物为只含2152个氨基酸残基的apoB-48, 缺失了apoB-100的N端同LDL受体结合的结构域。原因是在mRNA水平上将2153位的谷氨酰胺的密码子CAA中的C编辑成为U,形成一个终止密码子�UAA,结果使蛋白质的合成提前终止,合成一个新的相对分子质量约为250kDa的蛋白
人S 蛋白100B (S-100B) 酶联免疫分析 试剂盒使用说明书 本试剂仅供研究使用 目的：本试剂盒用于测定人血清，血浆及相关液体样本中S 蛋白100B (S-100B) 的含量。 实验原理： 本试剂盒应用双抗体夹心法测定标本中人 S 蛋白 100B (S-100B) 水平。用纯化的人 S 蛋白 100B (S-100B) 抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入 S 蛋白 100
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