Rat dopamine- Beta- hydroxylas

Rat Dopamine beta- hydroxylase, DBH ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

DBH,dopamine beta hydroxylase,DBM; dopamine beta-hydroxylase; dopamine beta-hydroxylase,dopamine beta-monooxygenase,

Search name

Rat DBH ELISA KIT ,Rat dopamine beta hydroxylase ELISA KIT ,Rat DBM ELISA KIT ,Rat dopamine beta-hydroxylase ELISA KIT ,Rat dopamine beta-hydroxylase ELISA KIT ,Rat dopamine beta-monooxygenase ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of rat dopamine beta-hydroxylase,Dopamine beta-hydroxylase concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dopamine beta-hydroxylase. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Dopamine beta-hydroxylase and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Dopamine beta-hydroxylase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Dopamine beta-hydroxylase in the samples is then determined by comparing the O.D. of the samples to the standard curve.