Rat carbonic anhydrase 2, CA-

Rat carbonic anhydrase 2, CA- 2 ELISA Kit
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Carbonic anhydrase 2,; Carbonate dehydratase II,Carbonic anhydrase II(CA-II),; Car2; AI131712; CAII; Ca2; Car-2; Ltw-5; Lvtw-5; CA II;
CA-II;

Search name
Rat carbonic anhydrase 2 ELISA Kit, Rat CA-2 ELISA Kit, Rat Carbonate dehydratase II ELISA Kit, Rat CA-II
ELISA Kit, Rat Car2 ELISA Kit, Rat AI131712 ELISA Kit, Rat Car-2 ELISA Kit, Rat Ltw-5 ELISA Kit, Rat
CA2 ELISA Kit, Rat Lvtw-5 ELISA Kit, Rat CA II ELISA Kit,

Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat Carbonic anhydrase 2 concentrations in serum, Plasma,
tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carbonic anhydrase 2. Standards or samples are
then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Carbonic
anhydrase 2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB
substrate solution is added to each well. Only those wells that contain Carbonic anhydrase 2, biotin-conjugated antibody and
enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid
solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Carbonic
anhydrase 2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.