- ￥2980
- LSM Bio
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELA-E0738r
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
大鼠
- 标记物：
Rat Activated Protein C,APC
- 样本：
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rat Activated Protein C, APC ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Vitamin K-dependent protein C,Blood coagulation factor XIV,Anticoagulant protein C,Autoprothrombin IIA,;
Search name
Rat Protein C ELISA Kit, Rat PROC ELISA Kit, Rat Vitamin K-dependent protein C ELISA Kit,Rat APC ELISA
KIT,Rat Activated Protein C ELISA KIT,
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat Vitamin K-dependent protein C concentrations in serum,
Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vitamin K-dependent protein C. Standards or
samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for
Vitamin K-dependent protein C and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated.
Then a TMB substrate solution is added to each well. Only those wells that contain Vitamin K-dependent protein C, biotin-conjugated
antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a
sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of
Vitamin K-dependent protein C in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Analysis of Protein Kinase C Function
Protein kinase C is a key element in signal transduction, cell regulation, and tumor promotion, influencing cellular responses to many stimuli at a variety of levels, including receptors, transduction systems, ion channels, and membrane pumps
Imaging Oscillations of Protein Kinase C Activity in Cells
The protein kinase C (PKC) family contains ten members that share a catalytic core but differ in the composition of their regulatory modules. The conventional PKCs are a subfamily whose regulatory domains bind to Ca
Isolation of an mRNA-Binding Protein Involved in C-to-U Editing
, which were coupled to cyanogen bromide (CNBr)-activated Sepharose. The methods are plasmid construction for in vitro transcription, affinity chromatography column preparation, protein purification by RNA affinity chromatography, and analysis of the purified protein.
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