Rat Pyruvate Kinase, M2- PK EL

Rat Pyruvate Kinase, M2- PK ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

M2- PK,PKM,CTHBP,HEL-S-30,OIP3,PK3,PKM2,TCB,THBP1,OIP-3,OPA-interacting protein 3,PK muscle type,cytosolic thyroid hormone-binding protein,epididymis secretory protein Li 30,p58,pyruvate kinase 2/3,pyruvate kinase PKM,pyruvate kinase isozymes M1/M2,pyruvate kinase muscle isozyme,thyroid hormone-binding protein 1,thyroid hormone-binding protein cytosolic,tumor M2-PK,pyruvate kinase muscle,Tumor M2-PK,Thyroid hormone-binding protein 1,THBP1,Pyruvate kinase muscle isozyme,Pyruvate kinase 2/3,p58,Cytosolic thyroid hormone-binding protein,CTHBP,Opa-interacting protein 3

Search name

Rat M2- PK ELISA KIT ,Rat M2PK ELISA KIT,Rat PKM ELISA KIT ,Rat CTHBP ELISA KIT ,Rat HEL-S-30 ELISA KIT ,Rat OIP3 ELISA KIT ,Rat PK3 ELISA KIT ,Rat PKM2 ELISA KIT ,Rat TCB ELISA KIT ,Rat THBP1 ELISA KIT ,Rat OIP-3 ELISA KIT ,Rat OPA-interacting protein 3 ELISA KIT ,Rat PK muscle type ELISA KIT ,Rat cytosolic thyroid hormone-binding protein ELISA KIT ,Rat epididymis secretory protein Li 30 ELISA KIT ,Rat p58 ELISA KIT ,Rat pyruvate kinase 2/3 ELISA KIT ,Rat pyruvate kinase PKM ELISA KIT ,Rat pyruvate kinase isozymes M1/M2 ELISA KIT ,Rat pyruvate kinase muscle isozyme ELISA KIT ,Rat thyroid hormone-binding protein 1 ELISA KIT ,Rat thyroid hormone-binding protein cytosolic ELISA KIT ,Rat tumor M2-PK ELISA KIT ,Rat pyruvate kinase muscle ELISA KIT ,Rat Tumor M2-PK ELISA KIT ,Rat Thyroid hormone-binding protein 1 ELISA KIT ,Rat THBP1 ELISA KIT ,Rat Pyruvate kinase muscle isozyme ELISA KIT ,Rat Pyruvate kinase 2/3 ELISA KIT ,Rat p58 ELISA KIT ,Rat Cytosolic thyroid hormone-binding protein ELISA KIT ,Rat CTHBP ELISA KIT ,Rat Opa-interacting protein 3 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of rat pyruvate kinase isozymes m1/m2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pyruvate kinase isozymes M1/M2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Pyruvate kinase isozymes M1/M2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Pyruvate kinase isozymes M1/M2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Pyruvate kinase isozymes M1/M2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.