- ￥2980
- LSM Bio
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELA-E0560r
- 2025年07月10日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
大鼠
- 标记物：
Rat Epidermal growth factor,EGF
- 样本：
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rat Epidermal growth factor, EGF ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Pro-epidermal growth factor(EGF), HOMG4; URG; beta-urogastrone; pro-epidermal growth factor; epidermal growth factor
Search name
Rat Pro-epidermal growth factor,EGF ELISA Kit, Rat EGF ELISA Kit, Rat HOMG4 ELISA Kit, Rat URG ELISA Kit, Rat
beta-urogastrone ELISA Kit, Rat Epidermal growth factor ELISA Kit,
Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat pro-epidermal growth factor,EGF concentrations in serum,
Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to EGF. Standards or samples are then added to the
appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for EGF and Avidin conjugated to
Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well.
Only those wells that contain EGF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The
enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of EGF in the samples is then determined by comparing the
O.D. of the samples to the standard curve.
PDF Manual download
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文献和实验表皮生长因子(epidermal growth factor, EGF)
表皮生长因子是一种小肽,由53个氨基酸残基组成, 是类EGF大家族的一个成员。EGF同应答细胞表面的特异受体结合,一旦结合,便促进受体二聚化并使细胞质位点磷酸化。被激活的受体至少可与5种具有不同信号序列的蛋白结合,进行信号转导。EGF能够广泛促进细胞的增殖。
Regulator of Epidermal Growth Factor Signaling: Sprouty
Sprouty was first discovered through its downregulatory effect on fibroblast growth factor (FGF) receptor pathway during tracheal development. Sprouty expression is also induced by the epidermal growth factor receptor (EGFR) cascade
Semiquantitative immunoblots of membrane protein-epidermal growth factor
Membrane proteins or cytokines are sometimes difficult to isolate and purify. Our group recently concentrated on epidermal growth factor (EGF) protein expression studies. Mature EGF was initially identified from mouse submaxillary gland extract
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